Sample Projects

Go back to:

Overview

Research publications

Or learn more on the general development and behaviour of frog tadpoles:

http://tadpoles.wp.st-andrews.ac.uk/

 

Here are some projects we have completed recently. More projects can be found at my colleagues’ website (tadpole research at Bristol).

Single hindbrain cells start tadpole swimming (Li & Soffe 2019. Front Cell Neurosci)

Unlike in invertebrates, it is rare to be able to change animal behaviour by activating single neurons in the vertebrate central nervous system. Among a large sample of neurons in frog tadpole hindbrain, we have recorded several excitatory neurons a single spike of which could start tadpole swimming repeatedly. Further analyses suggest that this initial excitation is amplified by other neurons of the same type before the whole swimming network becomes active. These powerful individual neurons may express the molecular marker of CHX10.

Self-absorbed comments: The presence of powerful individual neurons can ease our pain in understanding their functions in behaviour, since it is normally difficult to earmark and control the activity of neuronal populations of the same type. However, their existence also suggests big redundancies in the neuronal network, the evolutional advantages of which remain to be explored.

Red trace is an intracellular recording of the powerful hindbrain neuron (r.dIN1). Middle black trace (I inject., 9 trials) shows when a small current is used to evoke a single spike in the neuron, a bout of swimming activity is induced (vertical deflections in the blue trace, l.m.n.). Top diagram shows where the hindbrain neuron and swimming activity are recorded (see project below for CNS labelling).

Mechanosensory stimulation evokes acute concussion-like behaviour (Li 2017, eNeuro)

Most vertebrates have concussion responses when their heads are hit suddenly by heavy objects, rendering the animals momentarily motionless and often unconscious.  In Xenopus Laevis tadpoles, we find that concussion-like behaviour can be induced reliably by mechanosensory stimulation to the head skin.  The head skin stimulation then activates some cholinergic neurons in the brainstem to inhibit the tadpole motor circuit. The results provide a potential explanation why concussion in vertebrates often recovers spontaneously without sustaining clear physical injury to the brain and some acute symptoms of concussion can be a neurophysiological response to specific sensory stimulation.

Self-absorbed comments: Apparently animal forward movement needs to be stopped when its head bumps into solid objects. It remains an open question if concussion is an over-blown stopping response or the loss of motor control is just a consequence of concussion.

The top blue trace shows a hindbrain neuron is inhibited for more than two minutes after the head skin is stimulated electrically. Regular downward deflections are caused by current injections used to measure membrane resistance. The diagram below shows some unidentified hindbrain cholinergic interneurons (green) are activated by trigeminal mechanosensory cells (red) and mediate the concussion-like response.

Intrinsic properties enable motor program switches (Li 2015, J Neurosci)

Many neural circuits are capable of producing multiple, patterned outputs. This is because the synaptic connections among some network neurons are “ubiquitous” but susceptible to fast reconfiguration when sensory inputs or neuromodulatory states change. In this study, I have identified that certain ion channels in the tadpole motor circuit can quickly shift neuronal firing thresholds depending on network excitation levels. Neuron-type specific expression of the channels then allows selective recruitment/depression of different neurons, dynamic configuration of the circuit and potential switching between motor programmes (swimming and struggling in tadpoles).

Self-absorbed comments: In line with many invertebrate studies, this work shows that functional circuits can be very different from the synaptic connection maps. Also, it implies “ubiquitous” synaptic connections may be necessary for multiple functional networks.

A.The firing thresholds (numerals with arrows) of an inhibitory interneuron (aIN) are raised by hyperpolarised membrane potentials. B. Thresholds of an excitatory interneuron (dIN) are lowered by hyperpolarisation. I is the current injection used to alter membrane potential and evoke firing. RMP is the resting membrane potential.

A. The firing thresholds (numerals with arrows) of an inhibitory interneuron (aIN) are raised by hyperpolarised membrane potentials. B. Thresholds of an excitatory interneuron (dIN) are lowered by hyperpolarisation. I is the current injection used to alter membrane potential and evoke firing. RMP is the resting membrane potential.

The same neural circuit generates two rhythms (Li et al 2014, J Neurosci). Antiphase oscillations and synchrony are arguably the two most common neural rhythms in the CNS. We have found that both rhythms are intrinsically embedded in the same tadpole swimming circuit, which comprises of two half-centres coupled by reciprocal inhibition. The antiphase swimming rhythm is the stable output in normal conditions. Modelling predicts that synchrony can be converted to a stable regime by delaying the arrival of inhibition between the half-centres.

Self-absorbed comments: This research shows that the same central pattern generator based on post-inhibitory rebound can generate two different rhythms without circuit reconfiguration.

A. A commissural interneuron is recorded simultaneously with motor nerve activity from the opposite side. Red traces illustrate a brief bout of synchrony when the left and right activities appear in phase (c.f. out of phase activity after the bout). B. In a computer model built for tadpole swimming, synchrony (red traces) can be toggled on and off by injecting brief step currents into dINs.

A. A commissural interneuron is recorded simultaneously with motor nerve activity from the opposite side. Red traces illustrate a brief bout of synchrony when the left and right activities appear in phase (c.f. out of phase activity after the bout). B. In a computer model built for tadpole swimming, synchrony (red traces) can be toggled on and off by injecting brief step currents into dINs.

Normal tadpole swimming operates on rebound from reciprocal inhibition (Moult et al., 2013, Neuron). We wanted to identify which mechanism, rebound or pace-maker firing, dominates normal swimming activity. Using two fast silencing methods (optogenetics and large –DC injections into dINs, see other projects), we found that tadpole spinal activity on one side is dependent on the other side being active. Analyses show:
• the failure of activity result from depression of reciprocal inhibition
• pace-maker driven swimming-like rhythms requires a recovery period

Self-absorbed comments: This work lends strong support to the critical role for reciprocal inhibition in locomotor control proposed by Graham Brown (Brown’s hypothesis, Brown 1911, 1914).

A shows a tadpole expressing GFP-tagged ArCh constructs, an inhibitory reverse proton pump that can activated by yellow lights at 580 nm. ArCh was only expressed on the right hand side. B shows yellow light quickly switches off swimming monitored from the left hand motor nerve in an intensity-dependent manner. 1-10 are consecutive trials with or without light.

A shows a tadpole expressing GFP-tagged ArCh constructs, an inhibitory reverse proton pump that can activated by yellow lights at 580 nm. ArCh was only expressed on the right hand side. B shows yellow light quickly switches off swimming monitored from the left hand motor nerve in an intensity-dependent manner. 1-10 are consecutive trials with or without light.

The control of tadpole swimming frequency by excitation and inhibition (Li & Moult 2012, J Neurosci). Tadpole swimming rhythms are maintained either from rebound or pace-maker firing in the excitatory interneurons, dINs. By analysing how excitation and inhibition affect dIN rebound firing speed, we have identified that:
• Background excitation directly determines swimming frequency
• Strong phasic inhibition in certain cases can speed up swimming

Self-absorbed comments: It is rare in vertebrate central nervous system that changing the activity of single neurons can affect behavior. Fortuitously, single dIN firing can affect swimming. This makes testing many aspects of tadpole swimming control straightforward.

Injecting large –DC into a single dIN (blue traces and symbols) instantly reduces swimming frequency monitored from motor nerve bursts (m.n.).

Injecting large –DC into a single dIN (blue traces and symbols) instantly reduces swimming frequency monitored from motor nerve bursts (m.n.).

Identification of neurons that fire first in a behavior (Soffe et al., 2009, J Physiol). The neurons that fire first during certain behaviour can imply their critical, driving roles. Using loose-patch recordings, we recorded many neurons from single tadpoles. Analyses show that the earliest-firing neurons during a swimming cycle are the excitatory interneurons (dINs), located in the caudal hindbrain and rostral spinal cord region. While dIN EPSPs could directly drive the activity of other neurons, dIN firing itself appeared to rely on reciprocal inhibition (rebound).

Self-absorbed comments: It is uncommon to be able to find the neurons that fire first in a function mediated by the central nervous system. Though the approach we have used here is not ideal, the results are clear. The properties of dINs thus potentially hold many keys to swimming control.

A.Tadpole central nervous system (grey) with segmented swimming muscles. B. Relative timing of neuronal firing during a swimming cycle. Each red dot is averaged timing for a dIN. Black dots are for other types of neurons. Negative time means firing before motor nerve bursts (0 ms). Distance starts from midbrain (0 mm).

A.Tadpole central nervous system (grey) with segmented swimming muscles. B. Relative timing of neuronal firing during a swimming cycle. Each red dot is averaged timing for a dIN. Black dots are for other types of neurons. Negative time means firing before motor nerve bursts (0 ms). Distance starts from midbrain (0 mm).

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